李爽

李爽，1978年9月出生于黑龙江省，汉族女性，博士学位毕业于清华大学。现任华南理工大学生物科学与工程学院教授，研究方向为工业生物技术。

人物经历

教育经历

工作经历

2006.8-2007.6 广药集团广州拜迪生物医药有限公司，担任科研开发部部长。

2007-8-2010.8华南理工大学生物科学与工程学院讲师。

现任华南理工大学生物科学与工程学院教授。

主要成就

科研成就

科研项目

关于改善丙三醇脱水酶机理性失活的研究，国家自然科学基金青年科学基金项目。

科研成果

对酶的设计、表达和改性有深入认识，在定向进化技术的应用具有丰富的经验。所设计合成的可紫外/热诱导的E. coli自动裂解体系能够实现大肠杆菌胞内酶的表达和高通量筛选，且成本低廉、操作简单。且已成功应用于酶的定向进化工作，现已授权给瑞士的 DualSystems Biotech公司进行世界范围销售(货号：P08001)。该项工作还获得了第七届亚太生物化学工程会议的“Outstanding Poster Presentation”奖励。此外利用脱氧核糖核酸重组技术，建立了一套有效的蛋白结构功能单元的快速筛选/选择方法。该方法构思巧妙，不具有基因（或蛋白）特异性，不需要了解蛋白结构—功能关系，操作简单，是寻找蛋白可折叠单元的通用方法。该项工作已经受邀发表在Methods in Molecular Biology（Humana Press, a part of Springer Science）一书中。

（1）利用合成生物学的工具，指导改造或合成新型的或改良的生物学体系（细胞工厂），以生产生物能源燃料及生物医药化学品。

（2）通过代谢途径(网络)不断的改造和优化，系统提高目标产物产量，或降低毒害中间产物的产生与积累。

（3）利用蛋白/酶改造技术，有针对性的改造细胞工厂内关键蛋白/酶，提升反应效率。

专利

林章凛，李爽，蔡真, 病毒表面蛋白基因分解和表面蛋白的大规模制备，中国专利奖。

林章凛，李爽，陈勇，一种基于筛选方法获得具有独立结构/功能的蛋白片段的方法。

林章凛，李爽，徐丽华，依赖紫外诱导裂解基因的胞内酶高通量筛选体系。

林章凛，陈勇，李爽，一种基于选择方法获得具有独立结构/功能的蛋白片段的方法。中国专利

林章凛，徐丽华，李爽，任川，依赖热诱导裂解基因的胞内酶高通量筛选体系。

论文

1. Zhu M, Zhang L, Yang F, Cha Y, Li S*, Zhuo M, Huang S, Li J. A recombinant β-mannanase from Thermoanaerobacterium aotearoense SCUT27: Biochemical characterization and its thermostability improvement. J Agr Food Chem 2020, 68: 818-825.

2. Chen H, Zhu C, Zhu M, Xiong J, Ma H, Zhuo M, and Li S*. High production of valencene in Saccharomyces cerevisiae through metabolic engineering. Microb Cell FACT 2019, 18:195.

3. Yuan P, Dong J, Zhao W, Zhuo M, Li S*, Huang S, and Li J. Qualitative and quantitative assessment of genotoxins using SRRz lysis reporter under the control of a newly designed SOS responsive promoter in 埃希氏菌属 coli. RSC Advances. 2019, 9:35662-35670.

4. Huang S, Huang X, Li S, Zhu M, Zhuo M. MHC class I allele diversity in cynomolgus macaques of Vietnamese origin. PeerJ. 2019, 7:e7941.

5. Ye F, Liu S, Yang Y, Zhao T, Li S, Zhou T, et al. Identification of the major metabolites of (R)-沙丁胺醇 in human urine, plasma and feces using ultra high performance liquid chromatography coupled with quadrupole 时间of-flight 质量 spectrometry. J Sep Sci. 2019, 42:3200-8.

6. Ouyang, X,Cha Y, Li W, Zhu C, Zhu M, Li S*, Zhuo M, Huang S and Li J. Stepwise engineering of Saccharomyces cerevisiae to produce (+)-valencene and its related sesquiterpenes. RSC Advances, 2019, 9:30171-30181.

7. Xu T, Huang X, Li Z, Ki Lin C S, Li S*, Enhanced purification efficiency and thermal tolerance of Thermoanaerobacterium aotearoense β-Xylosidase thRough aggregation triggered by short peptides. J Agr Food Chem 2018, 66: 4182-4188.

8. Xu H, Tong N, Huang S, Hayat W, Fazal S, Li J, Li S, Yan J, Zhang Y, Simultaneous autotrophic removal of sulphate and nitrate at different voltages in a bioelectrochemical reactor (BER): Evaluation of degradation efficiency and characterization of microbial communities. Bioresour Technol 2018, 265:340-348.

9. Xu H, Tong N, Huang S, Zhou S, Li S, Li J, Zhang Y, Degradation of 2,4,6-三氯苯酚 and determination of bacterial community structure by micro-electrical stimulation with or without external organic 碳 source. Bioresour Technol 2018, 263:266-272.

10. Du C, Abdullah JJ, Greetham D, Fu D, Yu M, Ren L, Li S, Lu D. Valorization of food waste into biofertiliser and its field application. J Clean Prod 2018, 187:273-284.

11. Zhu M, Fan W, Cha Y, Yang X, Lai Z, Li S*, Wang, X. Dynamic cell responses in Thermoanaerobacterium sp. under hyperosmotic stress. Sci Rep, 2017, 7:10088.

12. Yang X, Zhu M, Huang X, Lin CSK, Wang J, Li S*, Valorisation of mixed bakery waste in non-sterilized 发酵 for l-lactic acid production by an evolved Thermoanaerobacterium sp. strain. Bioresource Technol, 2015, 198:47-54.

13. Zhu M, Lu Y, Wang J, Li S*, Wang X, 碳 catabolite repression and the related genes of ccpA, ptsH and hprK in Thermoanaerobacterium aotearoense. PLoS ONE, 2015, 10:e0142121. (JCR Q1, IF2.776)

14. Huang X, Li Z, Du C, Wang J, Li S*, Improved expression and characterization of a multi-domain xylanase from Thermoanaerobacterium aotearoense SCUT27 in 芽孢杆菌属 subtilis. J arg Food Chem, 2015, 63:6430-6439.

15. Yang F, Yang X, Li Z, Du C, Wang J, Li S*, Overexpression and characterization of a glucose-tolerant β-glucosidase from T. aotearoense with high specific activity for cellobiose. APPL Microbiol Biotechnol, 2015, 99:8903-8915.

16. Yang X, Huang A, Peng J, Wang J, Wang X, Lin Z*, Li S*, Self-assembly amphipathic peptides induce active enzyme aggregation that dramatically increases the operational stability of nitrilase, RSC Advances, 2014, 4: 60675-60684.

17. Lai Z, Zhu M, Yang X, Wang J, Li S* Optimization of key factors affecting 氢 production from SUGAR CANE bagasse by a thermophilic anaerobic pure culture. Biotechnol Biofuels 2014, 7:119. (JCR Q1, IF3.6.22)

18. Ai H, Zhang J, Yang M, Yu P, Li S, Zhu M, 越南盾 H, Wang S, Wang J: Draft Genome Sequence of an Anaerobic, Thermophilic Bacterium, Thermoanaerobacterium aotearoense SCUT27, Isolated from a Hot Spring in China. Genome Announc 2014, 2.

19. Yang X, Lai Z, Lai C, Zhu M, Li S*, Wang J*, Wang X: Efficient production of l-lactic acid by an engineered Thermoanaerobacterium aotearoense with broad substrate specificity. Biotechnol Biofuels 2013, 6:124.

20. Xu W, Chooi YH, Choi JW, Li S, Vederas JC, Da Silva NA, Tang Y: LovG: the thioesterase required for dihydromonacolin L release and lovastatin nonaketide synthase turnover in lovastatin biosynthesis. Angew Chem Int Ed Engl 2013, 52:6472-6475.

21. Li L, Ai H, Zhang S, Li S, Liang Z, Wu ZQ, Yang ST, Wang JF: Enhanced butanol production by coculture of Clostridium beijerinckii and Clostridium tyrobutyricum. Bioresour Technology 2013, 143:397-404.

22. Liang ZX, Li L, Li S, Cai YH, Yang ST, Wang JF: Enhanced propionic acid production from Jerusalem artichoke hydrolysate by immobilized Propionibacterium acidipropionici in a fibrous-bed bioreactor. Bioprocess Biosyst Eng 2012, 35:915-921. IF=1.82

23. Li S, Yang X, Yang S, Zhu M, Wang X: Technology prospecting on enzymes: Application, 推销理论 and engineering. Comput Struct Biotechnol J 2012, 2:e201209017.

24. Cai YH, Liang ZX, Li S, Zhu MJ, Wu ZQ, Yang ST, Wang JF: Bioethanol from 发酵 of cassava pulp in a fibrous-bed bioreactor using immobilized Δldh, a genetically engineered Thermoanaerobacterium aotearoense. Biotechnol Bioproc Eng 2012, 17:1270-1277.

25. Cai Y, Lai C, Li S, Liang Z, Zhu M, Liang S, Wang J: Disruption of lactate dehydrogenase through homologous recombination to improve bioethanol production in Thermoanaerobacterium aotearoense. Enzyme Microb Tech 2011, 48:155-161.

26.li S, Lai C, Cai Y, Yang X, Yang S, Zhu M, Wang J, Wang X: High efficiency 氢 production from glucose/xylose by the ldh-deleted Thermoanaerobacterium strain. Bioresour Technol 2010, 101:8718-8724.

教学成就

本科课程：《遗传学》《基因诊断与基因治疗》《细胞生物学实验》《生物化学实验》。

研究生课程：《科学实验设计与实验技能培训》。

社会活动

2012.9-2013.9加州大学洛杉矶分校(UCLA)化学与分子工程系访问学者。

参考资料

李爽.华南理工大学.2024-01-21

李爽.华南理工大学.2024-01-21